Abstract Background Predicting and proper ranking of canonical splice sites (SSs) is a challenging problem in bioinformatics and machine learning communities. Any progress in SSs recognition will lead to better understanding of splicing mechanism. We introduce several new approaches of combining a priori knowledge for improved SS detection. https://www.ealisboa.com/mega-save-2010-2013-CAMARO-ZL-STYLE-CARBON-FIBER-REAR-SPOILER-flash-save/